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Molecular Biology and Bioinformatics (B.Sc Botany)

Nucleic acids, gene expression, recombinant DNA basics, and biological databases and sequence analysis.

6 MonthsDuration 5Chapters22Topics

Syllabus · पाठ्यक्रम

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01Unit 1: Nucleic Acids 4 topics
  1. Griffith, Avery-MacLeod-McCarty and Hershey-Chase experiments
  2. Structure of DNA and RNA
  3. Organisation of prokaryotic and eukaryotic genomes
  4. Chloroplast and mitochondrial DNA
02Unit 2: Replication and Gene Expression 5 topics
  1. DNA replication and enzymes
  2. Transcription in prokaryotes and eukaryotes
  3. RNA processing
  4. Genetic code
  5. Translation
03Unit 3: Gene Regulation and Recombinant DNA Basics 4 topics
  1. lac and trp operons
  2. Restriction enzymes and vectors, Ti plasmid
  3. PCR and gene cloning (outline)
  4. Transgenic plants: Bt cotton, Golden rice
04Unit 4: Biological Databases 4 topics
  1. Introduction to bioinformatics
  2. Primary databases: GenBank, EMBL, DDBJ
  3. Secondary and specialised databases: UniProt, PROSITE, PDB, KEGG
  4. Data retrieval and file formats
05Unit 5: Sequence Analysis 5 topics
  1. Pairwise alignment: global and local
  2. Scoring matrices: PAM and BLOSUM
  3. BLAST programs
  4. Multiple sequence alignment
  5. Phylogenetic trees

About

You will be able to

  • Explain the experiments proving DNA as the genetic material and the structure of nucleic acids
  • Describe DNA replication, transcription, RNA processing and translation
  • Explain operon regulation and the basic tools of recombinant DNA technology and transgenic plants
  • Retrieve information from primary and secondary biological databases
  • Perform and interpret pairwise alignment, BLAST and multiple sequence alignment

Who it is for

  • B.Sc Botany students of Semester 5 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for M.Sc entrance (CUET-PG) in botany, biotechnology and bioinformatics

What you need

  • B.Sc Botany Semesters 1-4
  • Basic computer and internet use

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 5. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Do I need a computer at home?

Not necessary. Database and BLAST practice is done in the centre's computer lab.

Is there a test after the course?

Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.

Download the syllabus (PDF)

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