Molecular Biology and Bioinformatics (B.Sc Botany)

Nucleic acids, gene expression, recombinant DNA basics, and biological databases and sequence analysis.

6 महीनेअवधि 5Chapters22टॉपिक

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01Unit 1: Nucleic Acids 4 टॉपिक

Griffith, Avery-MacLeod-McCarty and Hershey-Chase experiments
Structure of DNA and RNA
Organisation of prokaryotic and eukaryotic genomes
Chloroplast and mitochondrial DNA

02Unit 2: Replication and Gene Expression 5 टॉपिक

DNA replication and enzymes
Transcription in prokaryotes and eukaryotes
RNA processing
Genetic code
Translation

03Unit 3: Gene Regulation and Recombinant DNA Basics 4 टॉपिक

lac and trp operons
Restriction enzymes and vectors, Ti plasmid
PCR and gene cloning (outline)
Transgenic plants: Bt cotton, Golden rice

04Unit 4: Biological Databases 4 टॉपिक

Introduction to bioinformatics
Primary databases: GenBank, EMBL, DDBJ
Secondary and specialised databases: UniProt, PROSITE, PDB, KEGG
Data retrieval and file formats

05Unit 5: Sequence Analysis 5 टॉपिक

Pairwise alignment: global and local
Scoring matrices: PAM and BLOSUM
BLAST programs
Multiple sequence alignment
Phylogenetic trees

परिचय

आप यह कर पाएँगे

Explain the experiments proving DNA as the genetic material and the structure of nucleic acids
Describe DNA replication, transcription, RNA processing and translation
Explain operon regulation and the basic tools of recombinant DNA technology and transgenic plants
Retrieve information from primary and secondary biological databases
Perform and interpret pairwise alignment, BLAST and multiple sequence alignment

यह किसके लिए है

B.Sc Botany students of Semester 5 (UP State Universities NEP 2020 common minimum syllabus)
Students preparing for M.Sc entrance (CUET-PG) in botany, biotechnology and bioinformatics

आपको क्या चाहिए

B.Sc Botany Semesters 1-4
Basic computer and internet use

सवाल

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 5. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Do I need a computer at home?

Not necessary. Database and BLAST practice is done in the centre's computer lab.

Is there a test after the course?

Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.