NDSS Academy

Bioinstrumentation Lab (B.Sc Zoology)

Hands-on use of microscopes, centrifuges, chromatography, electrophoresis and spectrophotometry.

6 MonthsDuration 5Chapters21Topics
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Syllabus · पाठ्यक्रम

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01Microscopy and Micrometry 5 topics
  1. Parts and care of the compound microscope
  2. Use of the oil immersion objective
  3. Calibration of the ocular micrometer with a stage micrometer
  4. Measuring cell size by micrometry
  5. Demonstration of phase contrast / fluorescence microscope
02pH Meter, Buffers and Centrifuge 4 topics
  1. Calibration of the pH meter with standard buffers
  2. Preparation of phosphate and acetate buffers
  3. Balancing tubes and operating a clinical centrifuge
  4. Separation of blood plasma / cell fractions by centrifugation
03Chromatography 4 topics
  1. Ascending paper chromatography of amino acids
  2. Thin layer chromatography of plant pigments
  3. Locating spots with ninhydrin / iodine
  4. Calculation of Rf values and identification
04Electrophoresis 4 topics
  1. Casting an agarose gel
  2. Loading DNA samples with tracking dye
  3. Running the gel and staining
  4. Viewing bands on a UV transilluminator, safety precautions
05Colorimetry and Spectrophotometry 4 topics
  1. Verification of the Beer-Lambert law
  2. Selection of filter / wavelength
  3. Estimation of protein by the biuret method
  4. Standard curve and estimation of an unknown

About

You will be able to

  • Use and care for a compound microscope, including the oil immersion objective and ocular micrometer
  • Calibrate a pH meter and prepare buffer solutions of a required pH
  • Operate a laboratory centrifuge safely with balanced tubes
  • Separate amino acids or pigments by paper and thin layer chromatography and calculate Rf values
  • Run an agarose gel and read DNA bands under UV with proper safety
  • Use a colorimeter / spectrophotometer to draw a standard curve and estimate an unknown

Who it is for

  • B.Sc Zoology students of Semester 3 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for the university practical exam and viva

What you need

  • Studying or completed the theory paper Molecular Biology, Bioinstrumentation and Biotechniques
  • Lab coat and practical record notebook

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 3. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Will I handle the instruments myself?

Yes. Each learner calibrates, runs and reads the instruments under the trainer's supervision.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.

Download the syllabus (PDF)

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