Your roadmap · आपका रोडमैप
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Admission & first meetingप्रवेश और पहली मुलाकातEnquire at your nearest NDSS centre, meet the teacher, fix the batch time and get your study material.
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Semester 3 — subject classesसेमेस्टर 3 — विषयवार कक्षाएँEvery theory subject of the term, unit by unit, with notes, numericals and doubt classes.
Molecular Biology, Bioinstrumentation and Biotechniques
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Labs, practicals & project workलैब, प्रैक्टिकल और प्रोजेक्टExperiments / jobs / project work as in the syllabus, with practical file and viva preparation.
Bioinstrumentation Lab
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Unit tests & mid-termयूनिट टेस्ट और मिड-टर्मA test after every unit and a mid-term in the university / board pattern.
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Semester 3 mock testमॉक टेस्टA full mock test of all subjects before the exam, with analysis.
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Semester 3 exam (university / board)सेमेस्टर / वार्षिक परीक्षाPrevious papers, important questions and full revision.
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Online tests & resultऑनलाइन टेस्ट और रिज़ल्टTake the tests online; your marks and result show in your student login and at the centre.
Syllabus · पाठ्यक्रम
2 subjects · 10 chapters · 53 topics
Molecular Biology, Bioinstrumentation and Biotechniques5 chapters · 6 Months
Subject page →+
01Unit 1: Nucleic Acids and DNA Replication
7 topics+
- Structure of DNA (Watson-Crick model), forms of DNA
- Types and structure of RNA
- Chargaff's rules
- Semi-conservative replication: Meselson-Stahl experiment
- Replication enzymes: helicase, primase, DNA polymerases, ligase, topoisomerase
- Leading and lagging strands, Okazaki fragments
- Replication in prokaryotes and eukaryotes, telomerase
02Unit 2: Transcription and Translation
7 topics+
- Prokaryotic RNA polymerase and sigma factor
- Initiation, elongation and termination of transcription
- Eukaryotic RNA polymerases and promoters
- Post-transcriptional processing: capping, tailing, splicing
- Genetic code and its properties
- Ribosome structure and tRNA charging
- Mechanism of translation and post-translational modification
03Unit 3: Gene Regulation and DNA Repair
6 topics+
- Operon concept (Jacob and Monod)
- lac operon: induction and catabolite repression
- trp operon: repression and attenuation
- Outline of gene regulation in eukaryotes
- DNA damage and mutation
- Photoreactivation, excision repair, mismatch repair
04Unit 4: Microscopy and Centrifugation
6 topics+
- Light microscope: magnification and resolving power
- Phase contrast and fluorescence microscopy
- Transmission and scanning electron microscopy
- Principle of centrifugation, RCF and rpm
- Differential and density gradient centrifugation
- Ultracentrifuge and sedimentation coefficient (Svedberg unit)
05Unit 5: Chromatography, Electrophoresis and Spectrophotometry
6 topics+
- Paper and thin layer chromatography, Rf value
- Column, ion-exchange and gel filtration chromatography
- Agarose gel and SDS-PAGE electrophoresis
- Beer-Lambert law
- Colorimeter and UV-visible spectrophotometer
- PCR and blotting techniques (outline)
Bioinstrumentation Lab5 chapters · 6 Months
Subject page →+
01Microscopy and Micrometry
5 topics+
- Parts and care of the compound microscope
- Use of the oil immersion objective
- Calibration of the ocular micrometer with a stage micrometer
- Measuring cell size by micrometry
- Demonstration of phase contrast / fluorescence microscope
02pH Meter, Buffers and Centrifuge
4 topics+
- Calibration of the pH meter with standard buffers
- Preparation of phosphate and acetate buffers
- Balancing tubes and operating a clinical centrifuge
- Separation of blood plasma / cell fractions by centrifugation
03Chromatography
4 topics+
- Ascending paper chromatography of amino acids
- Thin layer chromatography of plant pigments
- Locating spots with ninhydrin / iodine
- Calculation of Rf values and identification
04Electrophoresis
4 topics+
- Casting an agarose gel
- Loading DNA samples with tracking dye
- Running the gel and staining
- Viewing bands on a UV transilluminator, safety precautions
05Colorimetry and Spectrophotometry
4 topics+
- Verification of the Beer-Lambert law
- Selection of filter / wavelength
- Estimation of protein by the biuret method
- Standard curve and estimation of an unknown
About this course
You will be able to
- Complete Molecular Biology, Bioinstrumentation and Biotechniques syllabus with regular tests
- Complete Bioinstrumentation Lab syllabus with regular tests
Who it is for
- B.Sc Zoology students of Semester 3 (UP State Universities NEP 2020 common minimum syllabus)
- Students preparing for semester exams and for M.Sc / CUET-PG entrance in life sciences
What you need
- B.Sc Zoology Semester 1-2 (cell biology and basic chemistry)
- Class 12 Biology level knowledge
Online tests & result
Practice and unit tests online; marks and result show in your student login and at the centre.
Student login →
Questions
Which syllabus does this course follow?
It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 3. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.
Is this paper only theory?
This is the theory paper. Its companion Bioinstrumentation Lab covers hands-on use of the same instruments.
Is there a test after the course?
Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.
Will I handle the instruments myself?
Yes. Each learner calibrates, runs and reads the instruments under the trainer's supervision.
How are the practicals marked?
The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.
