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Experiments in Plant Physiology and Molecular Biology (B.Sc Botany)

Experiments on osmosis, transpiration, photosynthesis, enzyme activity and DNA isolation.

6 MonthsDuration 5Chapters17Topics
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Syllabus · पाठ्यक्रम

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01Water Relations 4 topics
  1. Osmosis by potato osmometer
  2. Plasmolysis in Rhoeo / Tradescantia leaf peel
  3. Osmotic potential by the plasmolytic method
  4. Imbibition in seeds
02Transpiration 4 topics
  1. Ganong's potometer
  2. Four-leaf experiment
  3. Cobalt chloride paper method
  4. Stomatal index and frequency
03Photosynthesis and Respiration 3 topics
  1. Paper chromatography of chloroplast pigments
  2. Oxygen evolution by Hydrilla and effect of CO2 / light
  3. Respiratory quotient with Ganong's respirometer
04Enzyme Activity 3 topics
  1. Catalase activity in plant tissue
  2. Amylase activity
  3. Effect of temperature and pH on enzyme activity
05Molecular Biology 3 topics
  1. DNA isolation from plant tissue (CTAB method)
  2. Agarose gel electrophoresis (demonstration)
  3. Quantification of DNA

About

You will be able to

  • Demonstrate osmosis, plasmolysis and imbibition and determine osmotic potential
  • Measure the rate of transpiration and calculate stomatal index
  • Separate chloroplast pigments and study factors affecting photosynthesis
  • Study the effect of temperature and pH on enzyme activity
  • Isolate DNA from plant tissue

Who it is for

  • B.Sc Botany students of Semester 5 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for the university practical exam and viva

What you need

  • Studying the Semester 5 theory papers on plant physiology and molecular biology
  • Lab coat and practical record

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 5. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Are results recorded in a practical file?

Yes. Each experiment is recorded with aim, principle, observation table, calculation and result for the university exam.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.

Download the syllabus (PDF)

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