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Gene Technology, Immunology and Computational Biology (B.Sc Zoology)

Recombinant DNA technology, immune system and responses, and biological databases and bioinformatics tools.

6 MonthsDuration 5Chapters28Topics

Syllabus · पाठ्यक्रम

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01Unit 1: Recombinant DNA Technology 5 topics
  1. Restriction endonucleases and DNA ligase
  2. Cloning vectors: plasmids, phage lambda, cosmids, BAC and YAC
  3. Steps of gene cloning
  4. Transformation and selection of recombinants (blue-white screening)
  5. Genomic and cDNA libraries
02Unit 2: Techniques and Applications of Gene Technology 6 topics
  1. Polymerase chain reaction (PCR)
  2. Sanger DNA sequencing
  3. Southern, Northern and Western blotting
  4. DNA fingerprinting
  5. Transgenic animals and gene therapy
  6. Biosafety and ethical issues
03Unit 3: The Immune System 5 topics
  1. Innate and adaptive immunity
  2. Cells of the immune system
  3. Primary and secondary lymphoid organs
  4. Antigens, haptens and adjuvants
  5. Antibody structure and classes
04Unit 4: Immune Responses 6 topics
  1. MHC and antigen presentation
  2. Humoral and cell mediated immune responses
  3. Complement system
  4. Hypersensitivity and autoimmunity
  5. Vaccines and types of immunity
  6. Antigen-antibody reactions: agglutination, precipitation, ELISA
05Unit 5: Computational Biology 6 topics
  1. Introduction to bioinformatics
  2. Primary and secondary databases: GenBank, EMBL, DDBJ, UniProt, PDB
  3. Sequence formats (FASTA)
  4. Pairwise and multiple sequence alignment
  5. BLAST and E-value
  6. Introduction to phylogenetic trees

About

You will be able to

  • Explain the tools and steps of recombinant DNA technology and gene cloning
  • Describe PCR, DNA sequencing, blotting and applications such as transgenic animals and DNA fingerprinting
  • Describe the cells and organs of the immune system and the structure and classes of antibodies
  • Explain humoral and cell mediated immunity, MHC, hypersensitivity and vaccines
  • Retrieve sequences from biological databases and interpret a BLAST result
  • Solve simple numericals on restriction site frequency and PCR amplification

Who it is for

  • B.Sc Zoology students of Semester 4 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for M.Sc entrance (CUET-PG) in zoology, biotechnology and life sciences

What you need

  • B.Sc Zoology Semester 3 molecular biology
  • Basic computer and internet use for the bioinformatics unit

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 4. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Do I need a computer for this paper?

Only for the bioinformatics unit; the centre's computer lab is used to practise NCBI and BLAST searches.

Is there a test after the course?

Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.

Download the syllabus (PDF)

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