Instrumental Analysis (B.Sc Chemistry)

Experiments with colorimetry, pH-metry, conductometry, potentiometry and chromatography.

6 MonthsDuration 5Chapters17Topics

Syllabus · पाठ्यक्रम

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01Colorimetry and Spectrophotometry 3 topics
  1. Determination of lambda max
  2. Verification of Beer-Lambert law (KMnO4 or CuSO4)
  3. Concentration of an unknown solution
02pH-metry 3 topics
  1. Calibration of pH meter with buffers
  2. Strong acid versus strong base
  3. Weak acid versus strong base and pKa
03Conductometry 4 topics
  1. Cell constant
  2. Strong acid versus strong base
  3. Weak acid versus strong base
  4. Mixture of strong and weak acids
04Potentiometry 3 topics
  1. Fe2+ versus K2Cr2O7 or Ce4+
  2. Acid-base titration with glass electrode
  3. First and second derivative plots
05Chromatography 4 topics
  1. Paper chromatography of amino acids or inks
  2. TLC of plant pigments
  3. Calculation of Rf values
  4. Column chromatography

About

You will be able to

  • Verify the Beer-Lambert law and find unknown concentrations by colorimetry
  • Perform pH-metric titrations and determine pKa of a weak acid
  • Perform conductometric titrations and locate end points graphically
  • Perform potentiometric redox titrations
  • Separate mixtures by paper and thin-layer chromatography

Who it is for

  • B.Sc Chemistry students of Semester 4 (UP State Universities, NEP 2020)
  • Students preparing for the B.Sc Chemistry practical examination and viva

What you need

  • Theory of Quantum Mechanics and Analytical Techniques
  • Volumetric analysis skills

Questions

Which syllabus does this lab course follow?

It follows the B.Sc Chemistry Semester 4 syllabus of the UP State Universities (NEP 2020 common minimum syllabus). Where your own university's syllabus differs, the centre follows your university syllabus.

How are students assessed at the centre?

The trainer watches each experiment and marks it on a rubric (set-up, observations, calculation and viva); viva-style questions revise the theory behind the experiments.

Will this help in the semester examination?

Yes. The experiments match the university practical examination, including the record file and viva.

Download the syllabus (PDF)

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