NDSS Academy

Techniques in Microbiology and Plant Pathology (B.Sc Botany)

Sterilisation, culture media, staining of microbes and study of diseased plant specimens.

6 महीनेअवधि 5Chapters21टॉपिक
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सिलेबस · पाठ्यक्रम

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01Laboratory Safety and Sterilisation 4 टॉपिक
  1. Good laboratory practices and biosafety
  2. Autoclave and hot air oven
  3. Laminar air flow and flame sterilisation
  4. Filtration sterilisation
02Culture Media and Isolation 5 टॉपिक
  1. Preparation of nutrient agar and potato dextrose agar
  2. Serial dilution technique
  3. Pour plate and spread plate
  4. Streak plate for pure culture
  5. Counting colonies (cfu)
03Staining of Microbes 4 टॉपिक
  1. Simple staining
  2. Gram staining
  3. Lactophenol cotton blue mount of fungi
  4. Observation under oil immersion
04Algae, Fungi and Lichens 4 टॉपिक
  1. Temporary mounts of Nostoc, Oedogonium, Spirogyra
  2. Rhizopus, Penicillium, Puccinia stages
  3. Types of lichen thallus
  4. Mycorrhizal roots (demonstration)
05Plant Disease Specimens 4 टॉपिक
  1. Mosaic and leaf curl diseases
  2. Citrus canker and bacterial blight
  3. Late blight, white rust, rust and smut
  4. Red rot of sugarcane

परिचय

आप यह कर पाएँगे

  • Follow laboratory safety and sterilise glassware and media using autoclave and hot air oven
  • Prepare culture media and isolate microbes by serial dilution, pour plate and streak plate
  • Perform simple and Gram staining of bacteria and stain fungi with lactophenol cotton blue
  • Prepare temporary mounts of algae, fungi and lichens
  • Identify common plant diseases from symptoms on specimens

यह किसके लिए है

  • B.Sc Botany students of Semester 1 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for the university practical exam and viva

आपको क्या चाहिए

  • Studying the theory paper Microbiology and Plant Pathology
  • Lab coat and practical record notebook

सवाल

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 1. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Will I culture bacteria myself?

Yes, safe non-pathogenic sources such as curd, soil and air plates are used under supervision.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.

Download the syllabus (PDF)

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