Gene Technology, Immunology and Computational Biology (B.Sc Zoology)

Recombinant DNA technology, immune system and responses, and biological databases and bioinformatics tools.

6 महीनेअवधि 5Chapters28टॉपिक

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01Unit 1: Recombinant DNA Technology 5 टॉपिक

Restriction endonucleases and DNA ligase
Cloning vectors: plasmids, phage lambda, cosmids, BAC and YAC
Steps of gene cloning
Transformation and selection of recombinants (blue-white screening)
Genomic and cDNA libraries

02Unit 2: Techniques and Applications of Gene Technology 6 टॉपिक

Polymerase chain reaction (PCR)
Sanger DNA sequencing
Southern, Northern and Western blotting
DNA fingerprinting
Transgenic animals and gene therapy
Biosafety and ethical issues

03Unit 3: The Immune System 5 टॉपिक

Innate and adaptive immunity
Cells of the immune system
Primary and secondary lymphoid organs
Antigens, haptens and adjuvants
Antibody structure and classes

04Unit 4: Immune Responses 6 टॉपिक

MHC and antigen presentation
Humoral and cell mediated immune responses
Complement system
Hypersensitivity and autoimmunity
Vaccines and types of immunity
Antigen-antibody reactions: agglutination, precipitation, ELISA

05Unit 5: Computational Biology 6 टॉपिक

Introduction to bioinformatics
Primary and secondary databases: GenBank, EMBL, DDBJ, UniProt, PDB
Sequence formats (FASTA)
Pairwise and multiple sequence alignment
BLAST and E-value
Introduction to phylogenetic trees

परिचय

आप यह कर पाएँगे

Explain the tools and steps of recombinant DNA technology and gene cloning
Describe PCR, DNA sequencing, blotting and applications such as transgenic animals and DNA fingerprinting
Describe the cells and organs of the immune system and the structure and classes of antibodies
Explain humoral and cell mediated immunity, MHC, hypersensitivity and vaccines
Retrieve sequences from biological databases and interpret a BLAST result
Solve simple numericals on restriction site frequency and PCR amplification

यह किसके लिए है

B.Sc Zoology students of Semester 4 (UP State Universities NEP 2020 common minimum syllabus)
Students preparing for M.Sc entrance (CUET-PG) in zoology, biotechnology and life sciences

आपको क्या चाहिए

B.Sc Zoology Semester 3 molecular biology
Basic computer and internet use for the bioinformatics unit

सवाल

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 4. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Do I need a computer for this paper?

Only for the bioinformatics unit; the centre's computer lab is used to practise NCBI and BLAST searches.

Is there a test after the course?

Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.