NDSS Academy

Molecular Biology, Bioinstrumentation and Biotechniques (B.Sc Zoology)

DNA replication, transcription, translation, gene regulation and principles of laboratory instruments and techniques.

6 महीनेअवधि 5Chapters32टॉपिक
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सिलेबस · पाठ्यक्रम

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01Unit 1: Nucleic Acids and DNA Replication 7 टॉपिक
  1. Structure of DNA (Watson-Crick model), forms of DNA
  2. Types and structure of RNA
  3. Chargaff's rules
  4. Semi-conservative replication: Meselson-Stahl experiment
  5. Replication enzymes: helicase, primase, DNA polymerases, ligase, topoisomerase
  6. Leading and lagging strands, Okazaki fragments
  7. Replication in prokaryotes and eukaryotes, telomerase
02Unit 2: Transcription and Translation 7 टॉपिक
  1. Prokaryotic RNA polymerase and sigma factor
  2. Initiation, elongation and termination of transcription
  3. Eukaryotic RNA polymerases and promoters
  4. Post-transcriptional processing: capping, tailing, splicing
  5. Genetic code and its properties
  6. Ribosome structure and tRNA charging
  7. Mechanism of translation and post-translational modification
03Unit 3: Gene Regulation and DNA Repair 6 टॉपिक
  1. Operon concept (Jacob and Monod)
  2. lac operon: induction and catabolite repression
  3. trp operon: repression and attenuation
  4. Outline of gene regulation in eukaryotes
  5. DNA damage and mutation
  6. Photoreactivation, excision repair, mismatch repair
04Unit 4: Microscopy and Centrifugation 6 टॉपिक
  1. Light microscope: magnification and resolving power
  2. Phase contrast and fluorescence microscopy
  3. Transmission and scanning electron microscopy
  4. Principle of centrifugation, RCF and rpm
  5. Differential and density gradient centrifugation
  6. Ultracentrifuge and sedimentation coefficient (Svedberg unit)
05Unit 5: Chromatography, Electrophoresis and Spectrophotometry 6 टॉपिक
  1. Paper and thin layer chromatography, Rf value
  2. Column, ion-exchange and gel filtration chromatography
  3. Agarose gel and SDS-PAGE electrophoresis
  4. Beer-Lambert law
  5. Colorimeter and UV-visible spectrophotometer
  6. PCR and blotting techniques (outline)

परिचय

आप यह कर पाएँगे

  • Explain the structure of DNA and RNA and the semi-conservative replication of DNA
  • Describe transcription, RNA processing and translation in prokaryotes and eukaryotes
  • Explain gene regulation using the lac and trp operons and outline DNA repair mechanisms
  • State the principles of light and electron microscopy and of centrifugation
  • Explain the principles of chromatography, electrophoresis and spectrophotometry
  • Solve simple numericals on base composition, Rf value, RCF and the Beer-Lambert law

यह किसके लिए है

  • B.Sc Zoology students of Semester 3 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for semester exams and for M.Sc / CUET-PG entrance in life sciences

आपको क्या चाहिए

  • B.Sc Zoology Semester 1-2 (cell biology and basic chemistry)
  • Class 12 Biology level knowledge

सवाल

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 3. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Is this paper only theory?

This is the theory paper. Its companion Bioinstrumentation Lab covers hands-on use of the same instruments.

Is there a test after the course?

Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.

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