Your roadmap · आपका रोडमैप
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Admission & first meetingप्रवेश और पहली मुलाकातEnquire at your nearest NDSS centre, meet the teacher, fix the batch time and get your study material.
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Semester 4 — subject classesसेमेस्टर 4 — विषयवार कक्षाएँEvery theory subject of the term, unit by unit, with notes, numericals and doubt classes.
Gene Technology, Immunology and Computational Biology
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Labs, practicals & project workलैब, प्रैक्टिकल और प्रोजेक्टExperiments / jobs / project work as in the syllabus, with practical file and viva preparation.
Gene Technology and Immunology Lab
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Unit tests & mid-termयूनिट टेस्ट और मिड-टर्मA test after every unit and a mid-term in the university / board pattern.
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Semester 4 mock testमॉक टेस्टA full mock test of all subjects before the exam, with analysis.
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Semester 4 exam (university / board)सेमेस्टर / वार्षिक परीक्षाPrevious papers, important questions and full revision.
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Online tests & resultऑनलाइन टेस्ट और रिज़ल्टTake the tests online; your marks and result show in your student login and at the centre.
Syllabus · पाठ्यक्रम
2 subjects · 9 chapters · 44 topics
Gene Technology, Immunology and Computational Biology5 chapters · 6 Months
Subject page →+
01Unit 1: Recombinant DNA Technology
5 topics+
- Restriction endonucleases and DNA ligase
- Cloning vectors: plasmids, phage lambda, cosmids, BAC and YAC
- Steps of gene cloning
- Transformation and selection of recombinants (blue-white screening)
- Genomic and cDNA libraries
02Unit 2: Techniques and Applications of Gene Technology
6 topics+
- Polymerase chain reaction (PCR)
- Sanger DNA sequencing
- Southern, Northern and Western blotting
- DNA fingerprinting
- Transgenic animals and gene therapy
- Biosafety and ethical issues
03Unit 3: The Immune System
5 topics+
- Innate and adaptive immunity
- Cells of the immune system
- Primary and secondary lymphoid organs
- Antigens, haptens and adjuvants
- Antibody structure and classes
04Unit 4: Immune Responses
6 topics+
- MHC and antigen presentation
- Humoral and cell mediated immune responses
- Complement system
- Hypersensitivity and autoimmunity
- Vaccines and types of immunity
- Antigen-antibody reactions: agglutination, precipitation, ELISA
05Unit 5: Computational Biology
6 topics+
- Introduction to bioinformatics
- Primary and secondary databases: GenBank, EMBL, DDBJ, UniProt, PDB
- Sequence formats (FASTA)
- Pairwise and multiple sequence alignment
- BLAST and E-value
- Introduction to phylogenetic trees
Gene Technology and Immunology Lab4 chapters · 6 Months
Subject page →+
01DNA Isolation and Gel Electrophoresis
4 topics+
- Isolation of DNA from onion / goat liver
- Spooling of DNA with chilled ethanol
- Purity check by A260/A280 ratio
- Agarose gel electrophoresis of DNA
02Haematology and Blood Grouping
4 topics+
- ABO and Rh blood grouping by slide method
- Haemoglobin estimation by Sahli's method
- Differential leucocyte count
- Safe handling and disposal of blood samples
03Immunological Tests
4 topics+
- Ouchterlony double immunodiffusion
- Widal / rapid kit tests (demonstration)
- Study of slides of lymphoid organs
- ELISA (demonstration)
04Bioinformatics Exercises
4 topics+
- Retrieving a gene sequence from NCBI GenBank
- Nucleotide and protein BLAST
- Multiple sequence alignment with Clustal Omega
- Drawing a simple phylogenetic tree
About this course
You will be able to
- Complete Gene Technology, Immunology and Computational Biology syllabus with regular tests
- Complete Gene Technology and Immunology Lab syllabus with regular tests
Who it is for
- B.Sc Zoology students of Semester 4 (UP State Universities NEP 2020 common minimum syllabus)
- Students preparing for M.Sc entrance (CUET-PG) in zoology, biotechnology and life sciences
What you need
- B.Sc Zoology Semester 3 molecular biology
- Basic computer and internet use for the bioinformatics unit
Online tests & result
Practice and unit tests online; marks and result show in your student login and at the centre.
Student login →
Questions
Which syllabus does this course follow?
It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 4. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.
Do I need a computer for this paper?
Only for the bioinformatics unit; the centre's computer lab is used to practise NCBI and BLAST searches.
Is there a test after the course?
Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.
Is blood collection safe in the lab?
Only sterile single-use lancets are used, under supervision, and all sharps and swabs go into proper disposal containers.
How are the practicals marked?
The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.
