Cytogenetics, Plant Breeding and Ecology Lab (B.Sc Botany)

Mitosis and meiosis preparations, breeding techniques, and quadrat and soil and water analysis.

6 MonthsDuration 5Chapters18Topics

Syllabus · पाठ्यक्रम

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01Mitosis 4 topics
  1. Fixation of onion root tips
  2. Hydrolysis and staining (aceto-carmine / aceto-orcein)
  3. Squash preparation
  4. Stages of mitosis and mitotic index
02Meiosis 3 topics
  1. Fixing young flower buds
  2. Anther smear preparation
  3. Identification of meiotic stages
03Plant Breeding Techniques 3 topics
  1. Emasculation, bagging and tagging
  2. Pollen viability test
  3. Hybridisation in a self-pollinated crop (demonstration)
04Genetics Problems 3 topics
  1. Monohybrid and dihybrid problems
  2. Gene interaction ratios
  3. Chi-square test
05Ecological Exercises 5 topics
  1. Minimum quadrat size by species-area curve
  2. Frequency, density and abundance by quadrat
  3. Soil pH, moisture and texture
  4. pH and dissolved oxygen of water
  5. Ecological adaptations of specimens

About

You will be able to

  • Prepare root tip squashes and identify stages of mitosis and calculate mitotic index
  • Prepare anther smears and identify stages of meiosis
  • Perform emasculation, bagging and tagging for hybridisation
  • Solve genetics problems and test ratios by chi-square
  • Carry out quadrat studies and simple soil and water analysis

Who it is for

  • B.Sc Botany students of Semester 6 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for the university practical exam and viva

What you need

  • Studying the Semester 6 theory papers on cytogenetics and ecology
  • Practical record notebook

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 6. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Can mitosis slides be prepared quickly?

Yes. Onion root tips grown in 3-4 days on water are enough; the trainer helps with fixing and squashing.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.

Download the syllabus (PDF)

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