Commercial Botany and Phytochemical Analysis Lab (B.Sc Botany)

Study of economic plant products and phytochemical tests and extraction of plant compounds.

6 MonthsDuration 5Chapters18Topics

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Syllabus · पाठ्यक्रम

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01Economic Plant Products 4 topics

Cereals, pulses and oil seeds
Fibres: cotton and jute
Beverages, spices and sugar
Timber, rubber and gums

02Microchemical Tests 4 topics

Starch in potato and wheat (iodine test)
Protein in pulses (biuret / xanthoproteic)
Oil in groundnut (Sudan III)
Section of wheat grain and cotton fibre

03Extraction of Plant Compounds 3 topics

Maceration and Soxhlet extraction
Essential oil by hydro-distillation (Clevenger)
Concentration of extract and yield calculation

04Phytochemical Screening 4 topics

Tests for alkaloids
Tests for flavonoids and phenolics / tannins
Tests for saponins, glycosides and steroids
Reporting results

05Chromatography of Plant Extracts 3 topics

Paper / TLC of leaf pigments
TLC of a plant extract
Calculation of Rf values

About

You will be able to

Identify economic plant products and state their botanical source and useful part
Perform microchemical tests for starch, protein and oil in plant materials
Prepare plant extracts by maceration or Soxhlet and essential oils by hydro-distillation
Carry out preliminary phytochemical screening of plant extracts
Separate plant pigments or extract components by thin layer chromatography

Who it is for

B.Sc Botany students of Semester 4 (UP State Universities NEP 2020 common minimum syllabus)
Students preparing for the university practical exam and viva

What you need

Studying the theory paper Economic Botany, Ethnomedicine and Phytochemistry
Lab coat and practical record

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 4. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Are chemicals handled safely?

Yes. Solvents and reagents are used in small amounts under supervision with gloves and goggles.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.