NDSS Academy

Gene Technology and Immunology Lab (B.Sc Zoology)

DNA isolation, immunological tests, blood grouping and use of bioinformatics databases.

6 महीनेअवधि 4Chapters16टॉपिक
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सिलेबस · पाठ्यक्रम

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01DNA Isolation and Gel Electrophoresis 4 टॉपिक
  1. Isolation of DNA from onion / goat liver
  2. Spooling of DNA with chilled ethanol
  3. Purity check by A260/A280 ratio
  4. Agarose gel electrophoresis of DNA
02Haematology and Blood Grouping 4 टॉपिक
  1. ABO and Rh blood grouping by slide method
  2. Haemoglobin estimation by Sahli's method
  3. Differential leucocyte count
  4. Safe handling and disposal of blood samples
03Immunological Tests 4 टॉपिक
  1. Ouchterlony double immunodiffusion
  2. Widal / rapid kit tests (demonstration)
  3. Study of slides of lymphoid organs
  4. ELISA (demonstration)
04Bioinformatics Exercises 4 टॉपिक
  1. Retrieving a gene sequence from NCBI GenBank
  2. Nucleotide and protein BLAST
  3. Multiple sequence alignment with Clustal Omega
  4. Drawing a simple phylogenetic tree

परिचय

आप यह कर पाएँगे

  • Isolate genomic DNA from plant or animal tissue and check its purity
  • Cast and run an agarose gel and interpret DNA bands
  • Determine ABO and Rh blood groups by the slide method following safety rules
  • Perform and interpret simple immunological tests such as Ouchterlony double diffusion and rapid kit tests
  • Retrieve sequences from NCBI and run BLAST and multiple sequence alignment

यह किसके लिए है

  • B.Sc Zoology students of Semester 4 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for the university practical exam and viva

आपको क्या चाहिए

  • Studying or completed the theory paper Gene Technology, Immunology and Computational Biology
  • Lab coat, gloves and practical record notebook

सवाल

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 4. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Is blood collection safe in the lab?

Only sterile single-use lancets are used, under supervision, and all sharps and swabs go into proper disposal containers.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.

Download the syllabus (PDF)

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