Your roadmap · आपका रोडमैप
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Admission & first meetingप्रवेश और पहली मुलाकातEnquire at your nearest NDSS centre, meet the teacher, fix the batch time and get your study material.
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Semester 5 — subject classesसेमेस्टर 5 — विषयवार कक्षाएँEvery theory subject of the term, unit by unit, with notes, numericals and doubt classes.
Plant Physiology, Metabolism and BiochemistryMolecular Biology and BioinformaticsExperiments in Plant Physiology and Molecular Biology
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Unit tests & mid-termयूनिट टेस्ट और मिड-टर्मA test after every unit and a mid-term in the university / board pattern.
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Semester 5 mock testमॉक टेस्टA full mock test of all subjects before the exam, with analysis.
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Semester 5 exam (university / board)सेमेस्टर / वार्षिक परीक्षाPrevious papers, important questions and full revision.
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Online tests & resultऑनलाइन टेस्ट और रिज़ल्टTake the tests online; your marks and result show in your student login and at the centre.
Syllabus · पाठ्यक्रम
3 subjects · 15 chapters · 63 topics
Plant Physiology, Metabolism and Biochemistry5 chapters · 6 Months
Subject page →+
01Unit 1: Plant-Water Relations
5 topics+
- Water potential and its components
- Diffusion, osmosis, imbibition, plasmolysis
- Absorption of water and ascent of sap
- Transpiration and its factors
- Mechanism of stomatal opening and closing
02Unit 2: Mineral Nutrition and Translocation
4 topics+
- Essential macro and micronutrients
- Deficiency symptoms
- Passive and active absorption of ions
- Phloem transport: pressure flow hypothesis
03Unit 3: Photosynthesis and Respiration
5 topics+
- Photosynthetic pigments and photosystems
- Light reactions and photophosphorylation
- Calvin cycle, C4 and CAM pathways, photorespiration
- Glycolysis, Krebs cycle and electron transport
- Respiratory quotient, fermentation
04Unit 4: Nitrogen Metabolism and Enzymes
5 topics+
- Biological nitrogen fixation and nitrogenase
- Nitrate and ammonia assimilation
- Classification and nomenclature of enzymes
- Michaelis-Menten kinetics and Km
- Enzyme inhibition
05Unit 5: Plant Growth Regulators and Development
5 topics+
- Auxins, gibberellins, cytokinins
- Abscisic acid and ethylene
- Photoperiodism and phytochrome
- Vernalisation
- Seed dormancy and germination
Molecular Biology and Bioinformatics5 chapters · 6 Months
Subject page →+
01Unit 1: Nucleic Acids
4 topics+
- Griffith, Avery-MacLeod-McCarty and Hershey-Chase experiments
- Structure of DNA and RNA
- Organisation of prokaryotic and eukaryotic genomes
- Chloroplast and mitochondrial DNA
02Unit 2: Replication and Gene Expression
5 topics+
- DNA replication and enzymes
- Transcription in prokaryotes and eukaryotes
- RNA processing
- Genetic code
- Translation
03Unit 3: Gene Regulation and Recombinant DNA Basics
4 topics+
- lac and trp operons
- Restriction enzymes and vectors, Ti plasmid
- PCR and gene cloning (outline)
- Transgenic plants: Bt cotton, Golden rice
04Unit 4: Biological Databases
4 topics+
- Introduction to bioinformatics
- Primary databases: GenBank, EMBL, DDBJ
- Secondary and specialised databases: UniProt, PROSITE, PDB, KEGG
- Data retrieval and file formats
05Unit 5: Sequence Analysis
5 topics+
- Pairwise alignment: global and local
- Scoring matrices: PAM and BLOSUM
- BLAST programs
- Multiple sequence alignment
- Phylogenetic trees
Experiments in Plant Physiology and Molecular Biology5 chapters · 6 Months
Subject page →+
01Water Relations
4 topics+
- Osmosis by potato osmometer
- Plasmolysis in Rhoeo / Tradescantia leaf peel
- Osmotic potential by the plasmolytic method
- Imbibition in seeds
02Transpiration
4 topics+
- Ganong's potometer
- Four-leaf experiment
- Cobalt chloride paper method
- Stomatal index and frequency
03Photosynthesis and Respiration
3 topics+
- Paper chromatography of chloroplast pigments
- Oxygen evolution by Hydrilla and effect of CO2 / light
- Respiratory quotient with Ganong's respirometer
04Enzyme Activity
3 topics+
- Catalase activity in plant tissue
- Amylase activity
- Effect of temperature and pH on enzyme activity
05Molecular Biology
3 topics+
- DNA isolation from plant tissue (CTAB method)
- Agarose gel electrophoresis (demonstration)
- Quantification of DNA
About this course
You will be able to
- Complete Plant Physiology, Metabolism and Biochemistry syllabus with regular tests
- Complete Molecular Biology and Bioinformatics syllabus with regular tests
- Complete Experiments in Plant Physiology and Molecular Biology syllabus with regular tests
Who it is for
- B.Sc Botany students of Semester 5 (UP State Universities NEP 2020 common minimum syllabus)
- Students preparing for M.Sc entrance and competitive exams with botany
What you need
- B.Sc Botany Semesters 1-4
- Basic chemistry of biomolecules
Online tests & result
Practice and unit tests online; marks and result show in your student login and at the centre.
Student login →
Questions
Which syllabus does this course follow?
It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Botany, Semester 5. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.
Is there a practical for this paper?
Yes. Experiments in Plant Physiology and Molecular Biology (same semester) covers osmosis, transpiration, photosynthesis and enzyme experiments.
Is there a test after the course?
Yes. Unit-wise multiple-choice tests and a final exam paper are included for semester exam practice.
Do I need a computer at home?
Not necessary. Database and BLAST practice is done in the centre's computer lab.
Are results recorded in a practical file?
Yes. Each experiment is recorded with aim, principle, observation table, calculation and result for the university exam.
How are the practicals marked?
The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.
