Gene Technology and Immunology Lab

B.Sc · Semester 4 — DNA isolation, immunological tests, blood grouping and use of bioinformatics databases.

6 MonthsDuration 1Subject 4Chapters OnlineTests & result

Your roadmap · आपका रोडमैप

  1. 1
    Admission & first meetingप्रवेश और पहली मुलाकात

    Enquire at your nearest NDSS centre, meet the teacher, fix the batch time and get your study material.

  2. 2
    Work planकार्य योजना

    Step by step with your guide / trainer.

    DNA Isolation and Gel ElectrophoresisHaematology and Blood GroupingImmunological TestsBioinformatics Exercises
  3. 3
    File, report & vivaफ़ाइल, रिपोर्ट और वाइवा

    Practical file / report checked, mock viva before the final one.

  4. 4
    Online tests & resultऑनलाइन टेस्ट और रिज़ल्ट

    Take the tests online; your marks and result show in your student login and at the centre.

Syllabus · पाठ्यक्रम

1 subject · 4 chapters · 16 topics

01DNA Isolation and Gel Electrophoresis 4 topics
  1. Isolation of DNA from onion / goat liver
  2. Spooling of DNA with chilled ethanol
  3. Purity check by A260/A280 ratio
  4. Agarose gel electrophoresis of DNA
02Haematology and Blood Grouping 4 topics
  1. ABO and Rh blood grouping by slide method
  2. Haemoglobin estimation by Sahli's method
  3. Differential leucocyte count
  4. Safe handling and disposal of blood samples
03Immunological Tests 4 topics
  1. Ouchterlony double immunodiffusion
  2. Widal / rapid kit tests (demonstration)
  3. Study of slides of lymphoid organs
  4. ELISA (demonstration)
04Bioinformatics Exercises 4 topics
  1. Retrieving a gene sequence from NCBI GenBank
  2. Nucleotide and protein BLAST
  3. Multiple sequence alignment with Clustal Omega
  4. Drawing a simple phylogenetic tree

About this course

You will be able to

  • Isolate genomic DNA from plant or animal tissue and check its purity
  • Cast and run an agarose gel and interpret DNA bands
  • Determine ABO and Rh blood groups by the slide method following safety rules
  • Perform and interpret simple immunological tests such as Ouchterlony double diffusion and rapid kit tests
  • Retrieve sequences from NCBI and run BLAST and multiple sequence alignment

Who it is for

  • B.Sc Zoology students of Semester 4 (UP State Universities NEP 2020 common minimum syllabus)
  • Students preparing for the university practical exam and viva

What you need

  • Studying or completed the theory paper Gene Technology, Immunology and Computational Biology
  • Lab coat, gloves and practical record notebook
Online tests & result Practice and unit tests online; marks and result show in your student login and at the centre. Student login →

Questions

Which syllabus does this course follow?

It follows the UP State Universities NEP 2020 common minimum syllabus for B.Sc Zoology, Semester 4. Where your own university's syllabus differs, the centre follows your university's syllabus and question pattern.

Is blood collection safe in the lab?

Only sterile single-use lancets are used, under supervision, and all sharps and swabs go into proper disposal containers.

How are the practicals marked?

The trainer watches each experiment and marks it on a rubric; the viva questions here prepare you for the university practical exam.

Download the syllabus (PDF)

Tell us who you are — your copy is made with your name, and our counsellor can help you choose the right course and centre.

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